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Organizado por Genomics Core Facility

RNAseq - How low can you go?

  • Fechas:

    Del 27/05/19 al 29/05/19

  • Lugar:

    Auditori PRBB, Barcelona, España (mapa)

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RNAseq- How low can you go?

Aim of this course is to prepare RNA-Seq libraries for lllumina sequencing by using the novel NEBilext Ultra ll Directional RNA kit for low input applications, not including single cells transcriptomics.

Throughout the course, we will be generating libraries from ultra-low as well as from normal amounts of RNA (1 ng to 50 ng) ¡nput to better understand the impact of input amounts and PCR cycles on the bioinformatics dealing with complexity, coverage  duplication rates and unìformity of libraries. The course participants will be guided with tips and tricks through the work flow. Finally, the bioinformatics analysis of lhe data will be discussed.

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